Subcellular Localization of N-acylphosphatidyl- Ethanolamine Synthase in Cotyledons of Cotton Seedlings
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Nucleotide sequence of cottonseed malate synthase.
We report the nucleotide sequence of a cDNA clone encoding S-K-L has been reported to be a peroxisomal trafficking signal the complete amino acid sequence of the glyoxysomal enzyme in mammals (6). malate synthase (MS) (EC 4.1.3.2) in cotton seeds. A cDNA library was constructed in UNI-ZAP (Stratagene) from ACKNOWLEDGEMENT poly(A) + RNA extracted from cotyledons of 48-h-grown seedlings and scree...
متن کاملCytochemical Localization of Malate Synthase in Glyoxysomes
Cytochemical staining techniques for microbodies (peroxisomes) are limited at present to the enzymes catalase and alpha-hydroxy acid oxidase, and neither technique can distinguish glyoxysomes from other microbodies. Described here is a procedure using ferricyanide for the cytochemical demonstration by light and electron microscopy of malate synthase activity in glyoxysomes of cotyledons from fa...
متن کاملCottonseed malate synthase : purification and immunochemical characterization.
Malate synthase (EC 4.1.3.2), an enzyme unique to the glyoxylate cycle, was purified to homogeneity from cotyledons of 72-hours, darkgrown cotton (Gossypium hirsutum L.) seedlings. Homogeneity of the enzyme was assessed by silver staining SDS-PAGE gels. Purification was accomplished by using a single buffer medium through six steps involving one ammonium sulfate fractionation and chromatography...
متن کاملIn Vitro Studies of Nitrate Reductase Activity in Cotton Cotyledons: Effects of Dowex 1-Cl and BSA.
Germinating cotton (Gossypium hirsutum L. cv. Deltapine 16) cotyledons developed two peaks of in vitro nitrate reductase activity; the first was stable in vitro and appeared 24 hours after imbibition; and the second, which was extremely labile in vitro, began to develop after the seedlings had emerged and developed chlorophyll. Nitrite reductase activity peaked only after the seedlings had emer...
متن کاملDistribution and development of nitrate reductase activity in germinating cotton seedlings.
Activity of nitrate reductase in roots and cotyledons of cotton seedings (Gossypium hirsutum L. cv. Deltapine 16) increased rapidly on germination, reaching a maximum after 1 day of imbibition. Thereafter, activity declined until emergence and greening of the cotyledons, when it again began to increase steadily. Germinating soybean (Glycine max (L.) Merrill cv. Merit) and sunflower (Helianthus ...
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